MiMeDB: Showing metabocard for 5,10-Methylenetetrahydromethanopterin (MMDBc0049857)

Microbial

Record Information

Version

2.0

Status

Detected and Quantified

Creation Date

2022-04-29 22:44:54 UTC

Update Date

2025-01-16 10:39:12 UTC

Metabolite ID

MMDBc0049857

Metabolite Identification

Common Name

5,10-Methylenetetrahydromethanopterin

Description

This compound belongs to the family of Alkyl Glycosides. These are lipids containing a glycosyl moiety (one or several units) linked to the hydroxyl group of a fatty alcohol.

Structure

MOL SDF PDB SMILES InChI

Synonyms

Not Available

Molecular Formula

C₃₁H₄₅N₆O₁₆P

Average Mass

788.6934

Monoisotopic Mass

788.262965934

IUPAC Name

Not Available

Traditional Name

Not Available

CAS Registry Number

Not Available

SMILES

Not Available

InChI Identifier

InChI=1S/C31H45N6O16P/c1-13-22-14(2)36(12-37(22)23-27(33-13)34-31(32)35-28(23)45)16-5-3-15(4-6-16)9-17(38)24(42)18(39)10-50-30-26(44)25(43)20(52-30)11-51-54(48,49)53-19(29(46)47)7-8-21(40)41/h3-6,13-14,17-20,22,24-26,30,38-39,42-44H,7-12H2,1-2H3,(H,40,41)(H,46,47)(H,48,49)(H4,32,33,34,35,45)/t13-,14+,17-,18+,19-,20+,22+,24-,25+,26+,30-/m0/s1

InChI Key

GBMIGEWJAPFSQI-CAFBYHECSA-N

Chemical Taxonomy

Functional Ontology

Not Available

Physical Properties

State

Not Available

Predicted Properties

Not Available

Spectra

Not Available

Chromatographic Retention Times and Retention Indices

Retention Times

Chromatography Type	Technique	Column	Deposition Date	Retention Time (s)
Liquid Chromatography	HILIC	Waters Acquity UPLC-BEH Amide column (eluted)	2025-10-24	768.1
Liquid Chromatography	HILIC	Accucore 150 Amide HILIC (eluted)	2025-10-24	653.6
Liquid Chromatography	Reversed-phase	Hypersil Gold 1.9 µm C18 (eluted)	2025-10-24	137.0
Liquid Chromatography	Reversed-phase	Agilent Zorbax Eclipse Plus C18 (2.1 mm x 150 mm, 3.5 um) (eluted)	2025-10-24	393.3
Liquid Chromatography	Reversed-phase	XBridge C18 3.5u 2.1x50 mm (eluted)	2025-10-24	391.8
Liquid Chromatography	Reversed-phase	Ascentis Express C18 (eluted)	2025-10-24	225.1
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (eluted)	2025-10-24	588.3
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-24	1085.0
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC (eluted)	2025-10-24	410.2
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (eluted)	2025-10-24	184.0
Liquid Chromatography	HILIC	Merck SeQuant ZIC-HILIC (eluted)	2025-10-24	721.0
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC HSS T3 C18 (eluted)	2025-10-24	202.7
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (eluted)	2025-10-24	79.5
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-24	755.7
Liquid Chromatography	HILIC	Merck SeQuant ZIC-pHILIC column (eluted)	2025-10-24	627.5
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (void)	2025-10-24	81.2
Liquid Chromatography	Reversed-phase	Waters ACQUITY UPLC BEH C18 (eluted)	2025-10-24	360.2
Liquid Chromatography	Reversed-phase	Kinetex Core-Shell C18 2.6 um, 3.0 x 100 mm, Phenomenex (eluted)	2025-10-24	832.1
Liquid Chromatography	Reversed-phase	Waters Atlantis T3 (2.1 x 150 mm, 5 um) (eluted)	2025-10-24	762.0

Retention Indices

Chromatography Type	Peak Label	SMILES	Stationary Phase	Deposition Date	Retention Index
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_1	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O[Si](C)(C)C)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	1054.59
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_2	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O[Si](C)(C)C)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	2270.76
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_3	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@@H](CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)O[Si](C)(C)C)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	3472.46
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_4	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O[Si](C)(C)C)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	1723.91
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_5	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O[Si](C)(C)C)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	3098.25
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_6	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O[Si](C)(C)C)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	3450.94
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_7	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O[Si](C)(C)C)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	1870.31
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_8	C[C@@H]1NC2=C(C(O[Si](C)(C)C)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	2379.23
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_9	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O[C@@H](CCC(=O)O)C(=O)O)O[Si](C)(C)C)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	1504.6
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_10	C[C@H]1[C@@H]2[C@@H](C)N(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)CN2C2=C([NH]C(=N)N=C2O)N1[Si](C)(C)C	standard non polar	2025-10-24	1194.29
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_11	C[C@@H]1NC2=C(C(O)=NC(=N[Si](C)(C)C)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	3345.92
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_12	C[C@@H]1NC2=C(C(O)=NC(=N)N2[Si](C)(C)C)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard non polar	2025-10-24	2534.21
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_1	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O[Si](C)(C)C)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	3150.78
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_2	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O[Si](C)(C)C)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	1259.25
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_3	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@@H](CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)O[Si](C)(C)C)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	1088.92
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_4	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O[Si](C)(C)C)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	2103.01
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_5	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O[Si](C)(C)C)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	1193.48
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_6	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O[Si](C)(C)C)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	2386.54
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_7	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O[Si](C)(C)C)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	2225.66
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_8	C[C@@H]1NC2=C(C(O[Si](C)(C)C)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	2910.11
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_9	C[C@@H]1NC2=C(C(O)=NC(=N)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O[C@@H](CCC(=O)O)C(=O)O)O[Si](C)(C)C)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	2043.94
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_10	C[C@H]1[C@@H]2[C@@H](C)N(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)CN2C2=C([NH]C(=N)N=C2O)N1[Si](C)(C)C	standard polar	2025-10-24	3065.09
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_11	C[C@@H]1NC2=C(C(O)=NC(=N[Si](C)(C)C)[NH]2)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	1772.58
Gas Chromatography	5,10-Methylenetetrahydromethanopterin_TMS_12	C[C@@H]1NC2=C(C(O)=NC(=N)N2[Si](C)(C)C)N2CN(C3=CC=C(C[C@H](O)[C@H](O)[C@H](O)CO[C@H]4O[C@H](COP(=O)(O)O[C@@H](CCC(=O)O)C(=O)O)[C@@H](O)[C@H]4O)C=C3)[C@H](C)[C@@H]12	standard polar	2025-10-24	1302.13

Biological Properties

Cellular Locations

Not Available

Biospecimen Locations

Feces

Tissue Locations

Not Available

Associated OMIM IDs

114500 (Colorectal cancer)
610247 (Eosinophilic esophagitis)

Human Proteins and Enzymes

Protein ID	Protein Name	Protein Type	Uniprot ID	Species
MMDBp0192412	Serine hydroxymethyltransferase, mitochondrial	Enzyme	P34897	Homo sapiens
MMDBp0192414	Serine hydroxymethyltransferase, cytosolic	Enzyme	P34896	Homo sapiens

Human Pathways

Pathways

Name	SMPDB/PathBank

Microbial Pathways

Pathways

Not Available

Metabolic Reactions

Reactions

This table shows at most 20 reactions. For the full list of associated reactions:

See All Associated Reactions

Reaction ID	Precursor	Product	Enzyme	Reaction Type	Data Source	Reference
MMDBr0024542	5,10-Methylenetetrahydromethanopterin	5,10-Methenyl-5,6,7,8-tetrahydromethanopterin	F420-dependent methylenetetrahydromethanopterin dehydrogenase	Dehydrogenation	RHEA	34755880
MMDBr0024543	5,10-Methylenetetrahydromethanopterin	5,10-Methenyl-5,6,7,8-tetrahydromethanopterin	F420-dependent methylenetetrahydromethanopterin dehydrogenase	Dehydrogenation	RHEA	34755880
MMDBr0024674	5-Methyl-5,6,7,8-tetrahydromethanopterin	5,10-Methylenetetrahydromethanopterin	5,10-methylenetetrahydromethanopterin reductase	Reduction	RHEA	34755880

Health Effects and Bioactivity

Health Outcome/Bioactivity	Metabolite Response/Effect	Evidence Type	Host and Biospecimen	Data Source	Reference

Microbial Sources

Superkingdom/Kingdom	Phylum	Total species
Archaea/Archaea	Euryarchaeota	20
Archaea	Euryarchaeota	4
Archaea/Archaea	nah	3
Bacteria/Eubacteria	Proteobacteria	1

Exposure Sources

Other Exposures

Source Type	Data Source	Reference

Host Biospecimen and Location

Host and Biospecimen	Status	Mean	Std	Min	Max	Units	Age	Sex	Health Condition	Data Source	Reference
Human Feces	Detected but not Quantified									HMDB

External Links

Not Available

General References

Magrane M: UniProt Knowledgebase: a hub of integrated protein data. Database (Oxford). 2011 Mar 29;2011:bar009. doi: 10.1093/database/bar009. Print 2011. [PubMed:21447597 ]

Showing metabocard for 5,10-Methylenetetrahydromethanopterin (MMDBc0049857)

MOL for #<Metabolite:0x00007f2e062a2278>

3D MOL for #<Metabolite:0x00007f2e062a2278>

3D SDF for #<Metabolite:0x00007f2e062a2278>

3D-SDF for #<Metabolite:0x00007f2e062a2278>

PDB for #<Metabolite:0x00007f2e062a2278>

3D PDB for #<Metabolite:0x00007f2e062a2278>

SMILES for #<Metabolite:0x00007f2e062a2278>

INCHI for #<Metabolite:0x00007f2e062a2278>

Structure for #<Metabolite:0x00007f2e062a2278>

3D Structure for #<Metabolite:0x00007f2e062a2278>